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In vitro cytotoxicity assay Cells (HFD) in the logarithmic growth phase were seeded into 96-well cell culture plates (6000 cells/well) and incubated overnight at 37 °C and 5 % CO 2
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Moreover, GPX4 in mitochondria exerts a more significant effect on cell resistance to ferroptosis than that exerted by GPX4 in the cytoplasm, and mitochondrial events seem to be final steps in the determination of whether cells undergo ferroptosis [287, 288]
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