bpc-157 español Select Size::300 gram

B12/MIC: Explained | AgelessRx

doi: 10.1038/s41598-021-87619-8, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 11.Komosinska-Vassev K, Olczyk P, Kaźmierczak J, Mencner L, Olczyk K

Catalog-Ref: A2F1

Select Size::300 gram

(3)制备Fmoc-Gly-Leu-Val-树脂： (3) Preparation of Fmoc-Gly-Leu-Val-resin: 在步骤(2)的Fmoc-Leu-Val-树脂中，加入脱帽试剂10～50℃反应5～30分钟，用真空泵抽干，重新加入脱帽试剂10～50℃反应10～60分钟，抽干，用异丙醇洗涤3 次，DMF洗涤3次，重蒸过的DMF洗涤2次，加入用DMF溶解的Fmoc-Gly-OH、DIEA、TBTU/HOBt或HBTU/HOBt或BOP/HOBt的混合物，10～50℃反应10～60分钟，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Gly-Leu-Val-树脂； In the Fmoc-Leu-Val-resin of step (2), add decapping reagent 10～50 ℃ and react for 5～30 minutes, drain with a vacuum pump, add decapping reagent 10～50 ℃ and react for 10～60 minutes, drain, Wash 3 times with isopropanol, DMF 3 times, redistilled DMF 2 times, add the mixture of Fmoc-Gly-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF, 10 React at ~50°C for 10 to 60 minutes, drain with a vacuum pump, wash with isopropanol 3 times, DMF for 3 times, double-distilled DMF for 2 times, and dry to obtain Fmoc-Gly-Leu-Val-resin