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Sterile transfer equipment: an unused sterile syringe and needle for moving diluent between vials Alcohol swabs for disinfecting the stoppers A permanent marker or blank label for the date and concentration A clean work surface , wiped down and cleared of unrelated items Concentration Math Concentration (mg/mL) = peptide (mg) ÷ diluent (mL) 10 mg vial 1 mL bacteriostatic water: 10 mg/mL 2 mL: 5 mg/mL 3 mL: about 3.33 mg/mL 20 mg vial 2 mL bacteriostatic water: 10 mg/mL 3 mL: about 6.67 mg/mL 4 mL: 5 mg/mL Which volume to use A larger diluent volume gives a weaker stock, so a given quantity of peptide sits in a bigger volume that can be measured with less relative error