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5-fold) is illustrated in Figure 2 and divided into the following steps: Distribution of 80 µl of diluent (water) to the 96 well flat-bottom plates Transfer of 20 µl of highly concentrated tartrazine into the first column Transfer of 20 µl of 5-fold diluted tartrazine into the second column Repeat One column contained only the diluent, functioning as a blank to eliminate background absorbance

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Together, these changes increase the risk of injury, infection, and tissue death (necrosis), contributing to a high rate of toe, foot, and lower leg amputations in people with diabetes