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PACK:Advanced - Pack of 3 (60 Tabs)

After incubation, mitochondria were pelleted (1 min at 16,000 × g ) and pellets were resuspended in 200 μL of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min