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Similarly, acute riboflavin depletion or deletion of riboflavin kinase, the enzyme responsible for the production of flavin co-factors, in murine macrophages, prevents TNF receptor 1-dependent activation of NADPH oxidase and production of reactive oxygen species, decreasing macrophage production of nitric oxide, IL-1β, IL-10, and CCL2, and increasing susceptibility to bacterial infection [181,182]