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, Li X., Yang H., Tang J., and Zhou S

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Despite the mechanistic challenges in creating an ideal mouse model of BRAF V600E -mutated LGG, it is feasible using the genetic engineering techniques with the following factors considered: 1) cell type-specific BRAF V600E mutation induced by either Cre-loxP or RCAS-TVA system, 2) selection of the appropriate genetic background of the mouse which can influence the development and progression of tumors, 3) monitoring of tumor progression by MRI to detect the presence and growth of tumors, 4) validation of the model by comparing its histopathological and molecular characteristics to human BRAF-mutated LGG, and 5) the validated mouse model can be used for preclinical research to test drugs to better understand the underlying biological mechanisms of BRAF-mutated LGG and this will pave the way for the identification of therapeutic targets to block tumor progression