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Cells were washed two times with prewarmed secretion buffer (138 mM NaCl, 4.5 mM KCl, 4.2 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 (2H 2 O), 2.5 mM CaCl 2 (2H 2 O), 1.2 mM MgCl 2 (6H 2 O), 10 mM HEPES