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In primary SS renal tubulointerstitial injury, CD163-positive cells and high expression of HIF-1α were reported, where HIF-1α could promote macrophage polarization and participate in renal tubular injury, and early intervention to release HIF-1α could improve renal function in patients (197)

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The dried samples were solubilized in a isopropanol–acetonitrile–water (50:25:25, vol:vol:vol, 100 µl) solution before liquid chromatography high-resolution mass spectrometry analyses