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In the subsequent study, they successfully incorporated three distinct UAAs in a single polypeptide using three different pairs of Mj TyrRS/ i tRNA Ty2 AUA , Ma PylRS/tRNA Pyl CUA , and Mm PylRS/tRNA Pyl UUA that were orthogonal to each other ( 3.2.6 Crafting a chimeric tRNA The development of chimeric tRNAs involves a rational design strategy that integrates diverse motifs from two or more tRNAs originating from distinct sources into a unified tRNA molecule
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