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After that, cells were treated with 5 mM concentration of AMPK activator or BTK binder or PHICS3 or DMSO control for 4 hrs in a fresh serum-free media before cells were lysed on ice in a lysis buffer (M-PERTM Mammalian Protein Extraction Reagent, HaltTM Protease and Phosphatase Inhibitor Cocktail (2X) and 50 mM NaF)
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