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Transwell migration and invasion assay Cells (8 × 10 4 ) were suspended in serum-free medium and seeded onto the upper chamber of Boyden chambers with an 8.0-μm pore size Boyden chambers (Corning, New York, USA) (for AGS and HGC-27 cells) or 12.0-μm pore size chambers (JET BIOFIL, Guangzhou, China) (for NUGC-4, KATO-III, and OCUM-1 cells), which were either coated with Matrigel (for invasion assays) or left uncoated (for migration assays)
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