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R&D Systems, Minneapolis, MN) in RPMI-1640 (Gibco, Detroit, MI) medium supplemented with 2% penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco) and 5% pooled normal human heat-inactivated and sterile-filtered AB serum (Innovative Research, Novi, MI) for 7 days, with a media change on day 5 with fresh M-CSF (10 ng/mL)
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Vascular function declines in parallel with gonadal aging in women and emerging evidence suggests this may also be the case for older adult men with low testosterone [116,117]
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