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Acute brain slice preparation Acute hippocampal slices used in single-cell electrophysiological recordings in experiments in which slices were treated with GAA or creatine in the presence or absence of gabazine were prepared as previously described 109 from C57BL/6J mice aged 5-7 weeks old

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3.3 Optimization of current FBS alternatives If the use of FBS could be replaced—at least in academic research—for culturing mammalian immortalized cell lines and primary cells, the overall demand for FBS could be reduced substantially (Wijerathna-Yapa et al., 2025