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This combination might constitute a potential breakthrough in the accurate diagnosis of the classic form of HT, as well as an antibody-negative chronic autoimmune thyroiditis (SN-CAT) [18]

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For flow cytometry, treated cells from 24-well plates were washed twice with Hank’s Balanced Salt Solution (HBSS), incubated with 50 nM TMRE for 30 min at 37 °C in the dark, washed twice with HBSS, trypsinized, and resuspended in HBSS supplemented with 10% FBS