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Protein Kinases Modulation Protein kinases such as ERK, AMPK, GSK3β, p38 MAPK, and PI3K/AKT can mediate Nrf2 phosphorylation which enhance Nrf2 stability, thereby promoting nuclear Nrf2 translocation and transactivation activity (Wei et al., 2013
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Despite the high transformation frequency, the spheroplast method failed to become an important and popular protocol, probably because of the tedious and complex procedure and because the transformants are embedded in regeneration agar, hindering replica plating
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